PCR and bioinformatics kit, includes DNA Extraction Module (12016408EDU) and Fish PCR Module (12016300EDU); for extraction and PCR amplification of up to 16 fish DNA and control samples (8 student workstations, 2 samples per workstation, 2–4 students per workstation); for educational use only.

Teach PCR, gel electrophoresis, and bioinformatics with this DNA barcoding lab activity in which students determine the species of a fish based on the DNA sequence of its cytochrome c oxidase I (COI) gene. Students first extract DNA from tissue, use PCR to amplify the DNA, and perform agarose gel electrophoresis to verify PCR products. Next, after receiving DNA sequencing results from a third party (sequencing services not included), students then use bioinformatics to genetically identify the species using the sequence obtained. The unique COI sequence produced for a given sample is considered its DNA barcode.

With this kit, you can teach your students how to:

• Perform DNA extraction, PCR amplification, and gel electrophoresis up to 16 fish DNA and control samples (8 student workstations, 2 samples per workstation, 2–4 students per workstation)
• Use DNA sequencing and bioinformatics to determine species identity
• Perform investigations in their area to examine biodiversity or see if marketplace substitution is occurring
• Contribute to global initiatives for barcoding life (Barcode of Life Database)