The Comparative Proteomics I Kit: Protein Profiler Module guides students through a laboratory-based scientific investigation. Students make predictions about their results in pre-lab activities using Internet databases and published phylogenetic information. They then employ protein electrophoresis to study protein structure and function, generating protein profiles from the muscles of both distantly and closely related species of fish. From their results, they compare the different species' profiles, construct cladograms (phylogenetic trees), and assign each organism a branch. Students can decide whether their results support their predictions.
- Explore evolution
- Study protein structure and function
- Use polyacrylamide gel electrophoresis (PAGE) to separate proteins by size
- Construct cladograms using data from their gel analysis
- Complete all activities in three 45-minute sessions
- Refill kit includes standards, actin and myosin standard, and DTT; for educational use only
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